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Detection of molecular markers associated with iron deficiency in sorghum (Sorghum bicolor L.) using bulked segregant analysis [electronic resource].

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Mahmoud, A. A

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Language: English Summary language: Arabic Description: p.833-844Other title:

تحديد المعلمات الوراثية الجزيئية المرتبطة بنقص الحديد في السورجم (Sorghum bicolor L.) باستخدام اختبار تحليل الانعزالات المتفارقة [Added title page title]

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Zagazig journal of agricultural research, 2005 v. 32 (3) [electronic resource].

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In: Zagazig Journal of Agricultural Research 2005.v.32(3)Summary: Two sorghum cultivars: TX 430 (iron defficiency tolerant) and IS 2219B (iron defficiency sensitive); were crossed to obtain FI plants that wereselfed to obtain F2 plants. Genomic DNA was extracted from the two constracting parents and their F1 and F2 (extreme tolerant and sensitive plants) in an attempt to find some DNA markers correlated with iron defficiency by means of bulked segregant analysis technique (BSA). Randomly ampilified polymorphic DNA of the polymerase chain reaction (RAPD - peR) was conducted using nine 10-mer primers. Using of the primers OPAOl, OP-A02, OP-A03, OP-A04 and OP-A06; one DNA fragment amplified for the tolerant parent and the tolerant F2 bulk. Such a DNA fragment did not appear in the sensitive parent, F1 and sensitive F2 bulk. These RAPD markers could be considered as reliable positive molecular markers linked with iron defficiency tolerance in sorghum i.e., OP-AOl, OP-A02, OP-A03, OP-A04 and OP-A06.

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Two sorghum cultivars: TX 430 (iron defficiency tolerant) and IS 2219B (iron defficiency sensitive); were crossed to obtain FI plants that wereselfed to obtain F2 plants. Genomic DNA was extracted from the two constracting parents and their F1 and F2 (extreme tolerant and sensitive plants) in an attempt to find some DNA markers correlated with iron defficiency by means of bulked segregant analysis technique (BSA). Randomly ampilified polymorphic DNA of the polymerase chain reaction (RAPD - peR) was conducted using nine 10-mer primers. Using of the primers OPAOl, OP-A02, OP-A03, OP-A04 and OP-A06; one DNA fragment amplified for the tolerant parent and the tolerant F2 bulk. Such a DNA fragment did not appear in the sensitive parent, F1 and sensitive F2 bulk. These RAPD markers could be considered as reliable positive molecular markers linked with iron defficiency tolerance in sorghum i.e., OP-AOl, OP-A02, OP-A03, OP-A04 and OP-A06.

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