Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma
Darwish,Samah.F.
Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma [electronic resource]. تقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى. - p.285-299.
Includes references.
The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays.
Summary in Arabic.
Cows--Diseases.
Semen--Analysis.
Cattle--Health.
Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma [electronic resource]. تقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى. - p.285-299.
Includes references.
The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays.
Summary in Arabic.
Cows--Diseases.
Semen--Analysis.
Cattle--Health.