Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma (Record no. 47761)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01763cab a2200289Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u194502 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110822s2011 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V57 No128 23 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Darwish,Samah.F. |
| 9 (RLIN) | 2639 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Assiut veterinary medical journal, 2011 v. 57 (128) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.285-299. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cows |
| General subdivision | Diseases. |
| 9 (RLIN) | 2640 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Semen |
| General subdivision | Analysis. |
| 9 (RLIN) | 2641 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cattle |
| General subdivision | Health. |
| 9 (RLIN) | 2642 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amin,A.S. |
| 9 (RLIN) | 2643 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Assiut Veterinary Medical Journal. |
| Related parts | 2011.v.57(128) |
| International Standard Serial Number | 1012-5973 |
| Control subfield | nnas |
| Record control number | u181356 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2011/AVMJ/5711/128/285.pdf">http://nile.enal.sci.eg/EALE/2011/AVMJ/5711/128/285.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.