Identification of zygotic and nucellar seedlings of Volkamer lemon, Rangpur lime and Cleopatra mandarin rootstocks using RAPD technique
Shaheen, M. A.
Identification of zygotic and nucellar seedlings of Volkamer lemon, Rangpur lime and Cleopatra mandarin rootstocks using RAPD technique [electronic resource]. تمييز الشتلات الجنسية والنيوسيلية لاصول الفولكامريانا وليمون الرانجبور ويوسفى الكليوباترا باستخدام تكنيك البوادئ العشوائية البلمرة. - P. 293-311.
Includes references.
The randomly amplified polymorphic DNA (RAPD-PCR) technique was used to identify and distinguish nucellar and zygotic 45-day-old seedlings of three citrus rootstocks namely Volkamer lemon (Citrus volkameriana Ten. & Pasq.), Rangpur lime (C. limonia, Osbeck), Cleopatra mandarin (C. reshni Hort. ex Tan.) with their mother trees. Primers OP-A2, OP-A8, OP-A18, OP-B3, OP-B4 and OP-B6 were used for identifying the nine rootstock genotypes. Seedlings classified as zygotic (sexual) have a different RAPD profile from that of the mother plant or nucellar (vegetative) seedlings. RAPD markers allowed the efficient identification of zygotic and nucellar seedlings.
Summary in Arabic.
Lemon--Genetics.
Lemon--Physiology.
Mandarin orange--Physiology.
Mandarin orange--Genetics.
RAPD technique.
Identification of zygotic and nucellar seedlings of Volkamer lemon, Rangpur lime and Cleopatra mandarin rootstocks using RAPD technique [electronic resource]. تمييز الشتلات الجنسية والنيوسيلية لاصول الفولكامريانا وليمون الرانجبور ويوسفى الكليوباترا باستخدام تكنيك البوادئ العشوائية البلمرة. - P. 293-311.
Includes references.
The randomly amplified polymorphic DNA (RAPD-PCR) technique was used to identify and distinguish nucellar and zygotic 45-day-old seedlings of three citrus rootstocks namely Volkamer lemon (Citrus volkameriana Ten. & Pasq.), Rangpur lime (C. limonia, Osbeck), Cleopatra mandarin (C. reshni Hort. ex Tan.) with their mother trees. Primers OP-A2, OP-A8, OP-A18, OP-B3, OP-B4 and OP-B6 were used for identifying the nine rootstock genotypes. Seedlings classified as zygotic (sexual) have a different RAPD profile from that of the mother plant or nucellar (vegetative) seedlings. RAPD markers allowed the efficient identification of zygotic and nucellar seedlings.
Summary in Arabic.
Lemon--Genetics.
Lemon--Physiology.
Mandarin orange--Physiology.
Mandarin orange--Genetics.
RAPD technique.