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Identification of zygotic and nucellar seedlings of Volkamer lemon, Rangpur lime and Cleopatra mandarin rootstocks using RAPD technique [electronic resource].

By:

Shaheen, M. A

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Language: English Summary language: Arabic Description: P. 293-311Other title:

تمييز الشتلات الجنسية والنيوسيلية لاصول الفولكامريانا وليمون الرانجبور ويوسفى الكليوباترا باستخدام تكنيك البوادئ العشوائية البلمرة [Added title page title]

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Journal of biological chemistry and environmental sciences, 2009 v. 4 (4) Part 2 [electronic resource].

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In: Journal of Biological Chemistry and Environmental Sciences 2009.v.4(4)2Summary: The randomly amplified polymorphic DNA (RAPD-PCR) technique was used to identify and distinguish nucellar and zygotic 45-day-old seedlings of three citrus rootstocks namely Volkamer lemon (Citrus volkameriana Ten. & Pasq.), Rangpur lime (C. limonia, Osbeck), Cleopatra mandarin (C. reshni Hort. ex Tan.) with their mother trees. Primers OP-A2, OP-A8, OP-A18, OP-B3, OP-B4 and OP-B6 were used for identifying the nine rootstock genotypes. Seedlings classified as zygotic (sexual) have a different RAPD profile from that of the mother plant or nucellar (vegetative) seedlings. RAPD markers allowed the efficient identification of zygotic and nucellar seedlings.

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The randomly amplified polymorphic DNA (RAPD-PCR) technique was used to identify and distinguish nucellar and zygotic 45-day-old seedlings of three citrus rootstocks namely Volkamer lemon (Citrus volkameriana Ten. & Pasq.), Rangpur lime (C. limonia, Osbeck), Cleopatra mandarin (C. reshni Hort. ex Tan.) with their mother trees. Primers OP-A2, OP-A8, OP-A18, OP-B3, OP-B4 and OP-B6 were used for identifying the nine rootstock genotypes. Seedlings classified as zygotic (sexual) have a different RAPD profile from that of the mother plant or nucellar (vegetative) seedlings. RAPD markers allowed the efficient identification of zygotic and nucellar seedlings.

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