PCR based detection of genes encoding virulence determinants in S. aureus strains isolated from bovine milk
Enany, M. E.
PCR based detection of genes encoding virulence determinants in S. aureus strains isolated from bovine milk [electronic resource]. استخدام اختبار البلمرة المتسلسل في تحديد الجينات الخاصة بضراوة الميكروب العنقودى الذهبي من البان الماشية. - P. 71-78.
Includes references.
A total of 84 S.aureus strains were isolated from subclinically mastitic bovine milk samples using traditional methods. Twenty eight S.aureus isolates were subjected to PCR for detection of coagulase gene, X region of protein A (spA) gene and panton valentine leukocidin (PVL) gene. All the isolates (100%) were positive for coagulase gene that showed no gene polymorphism, while 26 (92,8%) isolates were positive for spA gene that showed gene polymorphism with different sized amplicons..Only 3 (10.7%) isolates were positive for PVL gene. Multiplex PCR used for detection of enterotoxins genes (SEA, SEB, SEC and SED) in the same 28 isolates where 8 isolates (28.5%) were positive to SEA gene, one (3.5%) was positive for SEC gene, one (3.5%) was positive for both SEA and SEC genes and all isolates (100%) were negative for SEB gene. Hence 35.7% of these isolates possess enterotoxins genes.
Summary in Arabic.
Cattle--Diseases
Mastitis--Diseases
PCR based detection of genes encoding virulence determinants in S. aureus strains isolated from bovine milk [electronic resource]. استخدام اختبار البلمرة المتسلسل في تحديد الجينات الخاصة بضراوة الميكروب العنقودى الذهبي من البان الماشية. - P. 71-78.
Includes references.
A total of 84 S.aureus strains were isolated from subclinically mastitic bovine milk samples using traditional methods. Twenty eight S.aureus isolates were subjected to PCR for detection of coagulase gene, X region of protein A (spA) gene and panton valentine leukocidin (PVL) gene. All the isolates (100%) were positive for coagulase gene that showed no gene polymorphism, while 26 (92,8%) isolates were positive for spA gene that showed gene polymorphism with different sized amplicons..Only 3 (10.7%) isolates were positive for PVL gene. Multiplex PCR used for detection of enterotoxins genes (SEA, SEB, SEC and SED) in the same 28 isolates where 8 isolates (28.5%) were positive to SEA gene, one (3.5%) was positive for SEC gene, one (3.5%) was positive for both SEA and SEC genes and all isolates (100%) were negative for SEB gene. Hence 35.7% of these isolates possess enterotoxins genes.
Summary in Arabic.
Cattle--Diseases
Mastitis--Diseases