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PCR based detection of genes encoding virulence determinants in S. aureus strains isolated from bovine milk [electronic resource].

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Enany, M. E

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Language: English Summary language: Arabic Description: P. 71-78Other title:

استخدام اختبار البلمرة المتسلسل في تحديد الجينات الخاصة بضراوة الميكروب العنقودى الذهبي من البان الماشية [Added title page title]

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Zagazig veterinary journal, 2011 v. 39 (2) [electronic resource].

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In: Zagazig Veterinary Journal 2011.v.39(2)Summary: A total of 84 S.aureus strains were isolated from subclinically mastitic bovine milk samples using traditional methods. Twenty eight S.aureus isolates were subjected to PCR for detection of coagulase gene, X region of protein A (spA) gene and panton valentine leukocidin (PVL) gene. All the isolates (100%) were positive for coagulase gene that showed no gene polymorphism, while 26 (92,8%) isolates were positive for spA gene that showed gene polymorphism with different sized amplicons..Only 3 (10.7%) isolates were positive for PVL gene. Multiplex PCR used for detection of enterotoxins genes (SEA, SEB, SEC and SED) in the same 28 isolates where 8 isolates (28.5%) were positive to SEA gene, one (3.5%) was positive for SEC gene, one (3.5%) was positive for both SEA and SEC genes and all isolates (100%) were negative for SEB gene. Hence 35.7% of these isolates possess enterotoxins genes.

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A total of 84 S.aureus strains were isolated from subclinically mastitic bovine milk samples using traditional methods. Twenty eight S.aureus isolates were subjected to PCR for detection of coagulase gene, X region of protein A (spA) gene and panton valentine leukocidin (PVL) gene. All the isolates (100%) were positive for coagulase gene that showed no gene polymorphism, while 26 (92,8%) isolates were positive for spA gene that showed gene polymorphism with different sized amplicons..Only 3 (10.7%) isolates were positive for PVL gene. Multiplex PCR used for detection of enterotoxins genes (SEA, SEB, SEC and SED) in the same 28 isolates where 8 isolates (28.5%) were positive to SEA gene, one (3.5%) was positive for SEC gene, one (3.5%) was positive for both SEA and SEC genes and all isolates (100%) were negative for SEB gene. Hence 35.7% of these isolates possess enterotoxins genes.

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