Methods for genetic differentiation of Flavobacterium columnare isolated from some cultured freshwater fishes
El-Bouhy, Z.
Methods for genetic differentiation of Flavobacterium columnare isolated from some cultured freshwater fishes [electronic resource]. طرق التفريق الجينى لميكروب الفلافوبكتريم كولمنار المعزول من بعض اسماك المياه العذبة المستزرعة. - p.103-110.
Includes references.
Flavobacterium columnare is an important economical pathogen that affects freshwater fishes. This study aimed to investigate the genetic variations among the isolated F columnare from naturally infected Clarias gariepinus, Oreochromis niloticus and Cyprinus carpio using randomly amplified polymorphic DNA analysis (RAPD) PCR and Polyacrylamide gel electrophoresis (PAGE) in the presence of the sodium dodecylsulfate (SDS). The affected fishes with F. columnare suffered from hemorrhagic ulcer in the skin, severe fin rot especially at the caudal fin and respiratory manifestations. On ordal's selective media, the isolates showed characteristic yellowish rhizoids colonies. The isolated F. columnare from different fish species had no clear significant genetic variations. However, the total amount of the protein which is present in the cell wall of each isolate was completely different.
Summary in Arabic.
Freshwater fishes--Diseases.
Freshwater fishes--Microbioiolgy.
animal pathogenic bacteria
Flavobacterium columnare.
genetic variation
Methods for genetic differentiation of Flavobacterium columnare isolated from some cultured freshwater fishes [electronic resource]. طرق التفريق الجينى لميكروب الفلافوبكتريم كولمنار المعزول من بعض اسماك المياه العذبة المستزرعة. - p.103-110.
Includes references.
Flavobacterium columnare is an important economical pathogen that affects freshwater fishes. This study aimed to investigate the genetic variations among the isolated F columnare from naturally infected Clarias gariepinus, Oreochromis niloticus and Cyprinus carpio using randomly amplified polymorphic DNA analysis (RAPD) PCR and Polyacrylamide gel electrophoresis (PAGE) in the presence of the sodium dodecylsulfate (SDS). The affected fishes with F. columnare suffered from hemorrhagic ulcer in the skin, severe fin rot especially at the caudal fin and respiratory manifestations. On ordal's selective media, the isolates showed characteristic yellowish rhizoids colonies. The isolated F. columnare from different fish species had no clear significant genetic variations. However, the total amount of the protein which is present in the cell wall of each isolate was completely different.
Summary in Arabic.
Freshwater fishes--Diseases.
Freshwater fishes--Microbioiolgy.
animal pathogenic bacteria
Flavobacterium columnare.
genetic variation