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Methods for genetic differentiation of Flavobacterium columnare isolated from some cultured freshwater fishes [electronic resource].

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El-Bouhy, Z

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Language: English Summary language: Arabic Description: p.103-110Other title:

طرق التفريق الجينى لميكروب الفلافوبكتريم كولمنار المعزول من بعض اسماك المياه العذبة المستزرعة [Added title page title]

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Zagazig veterinary journal, 2011 v. 39 (1) [electronic resource].

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In: Zagazig Veterinary Journal 2011.v.39(1)Summary: Flavobacterium columnare is an important economical pathogen that affects freshwater fishes. This study aimed to investigate the genetic variations among the isolated F columnare from naturally infected Clarias gariepinus, Oreochromis niloticus and Cyprinus carpio using randomly amplified polymorphic DNA analysis (RAPD) PCR and Polyacrylamide gel electrophoresis (PAGE) in the presence of the sodium dodecylsulfate (SDS). The affected fishes with F. columnare suffered from hemorrhagic ulcer in the skin, severe fin rot especially at the caudal fin and respiratory manifestations. On ordal's selective media, the isolates showed characteristic yellowish rhizoids colonies. The isolated F. columnare from different fish species had no clear significant genetic variations. However, the total amount of the protein which is present in the cell wall of each isolate was completely different.

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Flavobacterium columnare is an important economical pathogen that affects freshwater fishes. This study aimed to investigate the genetic variations among the isolated F columnare from naturally infected Clarias gariepinus, Oreochromis niloticus and Cyprinus carpio using randomly amplified polymorphic DNA analysis (RAPD) PCR and Polyacrylamide gel electrophoresis (PAGE) in the presence of the sodium dodecylsulfate (SDS). The affected fishes with F. columnare suffered from hemorrhagic ulcer in the skin, severe fin rot especially at the caudal fin and respiratory manifestations. On ordal's selective media, the isolates showed characteristic yellowish rhizoids colonies. The isolated F. columnare from different fish species had no clear significant genetic variations. However, the total amount of the protein which is present in the cell wall of each isolate was completely different.

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