Improving the efficiency of the traditional method of camel chymosin extraction using ultrasound and a freeze-drying process | [electronic resource]
Ibrahim, A. H.
Improving the efficiency of the traditional method of camel chymosin extraction using ultrasound and a freeze-drying process [electronic resource] تحسين كفاءة طريقةالاستخلاص التقليدية لكيموسين الابل باستخدام الموجات فوق الصوتية وعملية التجفيف بالتجميد - p. 165-173.
Includes bibliographic reference
Many problems are associated with the traditional extracting methods of camel chymosin; they are costly, time-consuming,
and have low concentrations of chymosin. This study aimed to increase the efficiency of the traditional methods of camel
chymosin production. Two experiments were carried out; the first experiment was implemented using dried camel abomasum
as a powder and strips (4-7mm), and exposed to ultrasound exposure time at (20, 40, and 80 min). The chymosin extracted
using the dried powder with ultrasound exposure time at 80 min treatments caused a higher significant increase in milk
clotting activity and lower proteolytic activity compared to the strips and control treatments. The second experiment
was implemented using the freeze-dried process for both chymosin extracted from the strips and powder treatments,
which was selected as a higher in total clotting activity and chymosin yield. Upon comparison to the traditional samples,
the freeze-dried treatments caused a higher significant increase in specific milk-clotting time and MCA/PA ratio
by approximately 4.2 fold and 24.0 fold, respectively. Moreover, the freeze-dried process caused a significant increase
in chymosin yield, curd viscosity, firmness, and reduced the syneresis of milk curd. Furthermore, the results confirmed
that the viscosimetric measurement can be used as a new indicator to predict the cutting time of camel milk curd.
The use of ultrasound with the freeze-dried process can be recommended as an appropriate treatment for the extraction
of camel chymosin.
Keywords: Camel Chymosin, Ultrasound, Freeze-drying, Clotting activity, Proteolytic activity.
Summary in Arabic
Improving the efficiency of the traditional method of camel chymosin extraction using ultrasound and a freeze-drying process [electronic resource] تحسين كفاءة طريقةالاستخلاص التقليدية لكيموسين الابل باستخدام الموجات فوق الصوتية وعملية التجفيف بالتجميد - p. 165-173.
Includes bibliographic reference
Many problems are associated with the traditional extracting methods of camel chymosin; they are costly, time-consuming,
and have low concentrations of chymosin. This study aimed to increase the efficiency of the traditional methods of camel
chymosin production. Two experiments were carried out; the first experiment was implemented using dried camel abomasum
as a powder and strips (4-7mm), and exposed to ultrasound exposure time at (20, 40, and 80 min). The chymosin extracted
using the dried powder with ultrasound exposure time at 80 min treatments caused a higher significant increase in milk
clotting activity and lower proteolytic activity compared to the strips and control treatments. The second experiment
was implemented using the freeze-dried process for both chymosin extracted from the strips and powder treatments,
which was selected as a higher in total clotting activity and chymosin yield. Upon comparison to the traditional samples,
the freeze-dried treatments caused a higher significant increase in specific milk-clotting time and MCA/PA ratio
by approximately 4.2 fold and 24.0 fold, respectively. Moreover, the freeze-dried process caused a significant increase
in chymosin yield, curd viscosity, firmness, and reduced the syneresis of milk curd. Furthermore, the results confirmed
that the viscosimetric measurement can be used as a new indicator to predict the cutting time of camel milk curd.
The use of ultrasound with the freeze-dried process can be recommended as an appropriate treatment for the extraction
of camel chymosin.
Keywords: Camel Chymosin, Ultrasound, Freeze-drying, Clotting activity, Proteolytic activity.
Summary in Arabic