Aِlternative method for evaluation of infectious bovine rhinotracheitis, bovine viral diarrhea and parainfluenza-3 vaccines by using elisa testabstract (Record no. 54727)

MARC details
000 -LEADER
fixed length control field 02471cab a2200313Ia 45 0
001 - CONTROL NUMBER
control field u202902
003 - CONTROL NUMBER IDENTIFIER
control field SIRSI
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 120926s2010 ua ss b eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EAL
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
Language code of summary or abstract ara
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) ART ZVJ V38 No3 17
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Barghoot, W. M.
240 10 - UNIFORM TITLE
Uniform title Zagazig veterinary journal, 2010 v. 38 (3)
Medium [electronic resource].
245 10 - TITLE STATEMENT
Title Aِlternative method for evaluation of infectious bovine rhinotracheitis, bovine viral diarrhea and parainfluenza-3 vaccines by using elisa testabstract
Medium [electronic resource].
246 15 - VARYING FORM OF TITLE
Title proper/short title طريقة بديلة لتقييم كفاءة اللقاحات المضادة لامراض التهاب الانف والقصبة الهوائية المعدي والاسهال الفيروسي في الابقار والبارا انفلونزا 3- باستخدام اختبار الاليزا.
300 ## - PHYSICAL DESCRIPTION
Extent p.157-163
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes references.
520 ## - SUMMARY, ETC.
Summary, etc. The present study was designed to evaluate respiratory disease vaccines by in vitro method using specific hyperimmune sera which were prepared in rabbits and a sandwich ELISA was done to detect the titration of the vaccine (potency ratio test), and comparing these results with those obtained from animal testing of the same batches of the vaccine. Identity test was carried out on 3 prescriptions for each harvested virus and examined using fluorescent microscope. Fifteen Boscat rabbits and inactivated combined vaccines containing IBR, BVD and PI-3 viruses were used to prepare hyperimmune sera against IBR, BVD and PI-3 antigens. A capture (sandwich) ELISA was carried out to detect the potency of vaccine which in tum was compared with that obtained by the traditional method. We find that the in vitro method in the Laboratory using specific hyperimmune serum and sandwich ELISA is more economic, time and effort saving, other than the old method through injection in farm animals and using the separated serum in titration of antibody response which takes a lot oftime, money and effort.
546 ## - LANGUAGE NOTE
Language note Summary in Arabic.
596 ## -
-- 1
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Respiratory organs
General subdivision Diseases.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Enzyme-linked immunosorbent assay.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Veterinary vaccines.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Influenza vaccines.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Saad, M. A.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sayed,Magda M.
773 0# - HOST ITEM ENTRY
Title Zagazig Veterinary Journal.
Related parts 2010.v.38(3)
International Standard Serial Number 1110-1458
Control subfield nnas
Record control number u189860
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://nile.enal.sci.eg/EALE/2010/ZVJ/3810/3/151.pdf">http://nile.enal.sci.eg/EALE/2010/ZVJ/3810/3/151.pdf</a>
Public note Full Text Article.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Articles
Source of classification or shelving scheme Library of Congress Classification

No items available.


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