Aِlternative method for evaluation of infectious bovine rhinotracheitis, bovine viral diarrhea and parainfluenza-3 vaccines by using elisa testabstract [electronic resource].

Includes references.

The present study was designed to evaluate respiratory disease vaccines by in vitro method using specific hyperimmune sera which were prepared in rabbits and a sandwich ELISA was done to detect the titration of the vaccine (potency ratio test), and comparing these results with those obtained from animal testing of the same batches of the vaccine. Identity test was carried out on 3 prescriptions for each harvested virus and examined using fluorescent microscope. Fifteen Boscat rabbits and inactivated combined vaccines containing IBR, BVD and PI-3 viruses were used to prepare hyperimmune sera against IBR, BVD and PI-3 antigens. A capture (sandwich) ELISA was carried out to detect the potency of vaccine which in tum was compared with that obtained by the traditional method. We find that the in vitro method in the Laboratory using specific hyperimmune serum and sandwich ELISA is more economic, time and effort saving, other than the old method through injection in farm animals and using the separated serum in titration of antibody response which takes a lot oftime, money and effort.

Summary in Arabic.

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