Effect of cumulus cells on canine oocyte vitrification using DAP213 cryotop method (Record no. 60968)
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000 -LEADER | |
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fixed length control field | 02059cab a2200301Ia 45 0 |
001 - CONTROL NUMBER | |
control field | u212189 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | SIRSI |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 160817s2014 ua ss b eng d |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | EAL |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | engbara |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V60 NO142 7 |
100 1# - MAIN ENTRY--PERSONAL NAME | |
Personal name | Abdel-Ghani, M. A. |
9 (RLIN) | 416 |
240 10 - UNIFORM TITLE | |
Uniform title | Assiut veterinary medical journal, 2014 v. 60 (142) |
Medium | [electronic resource]. |
245 10 - TITLE STATEMENT | |
Title | Effect of cumulus cells on canine oocyte vitrification using DAP213 cryotop method |
Medium | [electronic resource]. |
246 15 - VARYING FORM OF TITLE | |
Title proper/short title | تأثير الخلايا الركامية على تزجيج بويضات الكلاب باستخدام محلولDAP213 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | p.46-53 |
504 ## - BIBLIOGRAPHY, ETC. NOTE | |
Bibliography, etc. note | Includes references. |
520 ## - SUMMARY, ETC. | |
Summary, etc. | The present study investigated the influence of cumulus cells on vitrification of canine germinal vesicle stage oocytes, and assessed the ability of vitrified -warmed oocytes for further maturation in vitro. Selected cumulus-oocyte complexes (COCs) or cumulus-free oocytes or partially denuded were vitrified in DAP 213 solution. The vitrified-warmed oocytes were observed morphologically, and the plasma integrity was assessed. The viable oocytes in each group were cultured in TCM-199 for 72 h at 38.5 oc in 5% C02, 5% 02 and 90% N2. All media were supplemented with 10% ofFCS, 50 ng/mL of EGF, 2 Ug/mL of estradiol-17?, 0.1 IU/mL ofhCG, 0.1 IU/mL of FSH, 0.25 mM of pyruvic acid, 100 UM of ?-mercaptoethanol, and antibiotics. The viability of vitrified-warmed intact oocytes (52.8%) was higher than denuded oocytes (33.9%, P < 0.05), however, there is no differences between the partially denuded oocytes (43.9%) and denuded oocytes (33.9%, P > 0.05). |
546 ## - LANGUAGE NOTE | |
Language note | Summary in Arabic. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Dogs |
General subdivision | Reproduction |
9 (RLIN) | 94 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Dogs |
General subdivision | Germplasm resources |
-- | Cryopreservation |
9 (RLIN) | 94 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Oocytin |
9 (RLIN) | 417 |
650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | cumulus oophorus |
9 (RLIN) | 418 |
700 1# - ADDED ENTRY--PERSONAL NAME | |
Personal name | Suzuki, H. |
9 (RLIN) | 419 |
773 0# - HOST ITEM ENTRY | |
Title | Assiut Veterinary Medical Journal. |
Related parts | 2014.v.60(142) |
International Standard Serial Number | 1012-5973 |
Control subfield | nnas |
Record control number | u181356 |
856 40 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/142/46.pdf">http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/142/46.pdf</a> |
Public note | Full Text Article. |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Articles |
Source of classification or shelving scheme | Library of Congress Classification |
No items available.