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Effect of cumulus cells on canine oocyte vitrification using DAP213 cryotop method [electronic resource].

By:

Abdel-Ghani, M. A

Contributor(s):

Suzuki, H

Language: engbara Description: p.46-53Other title:

تأثير الخلايا الركامية على تزجيج بويضات الكلاب باستخدام محلولDAP213 [Added title page title]

Uniform titles:

Assiut veterinary medical journal, 2014 v. 60 (142) [electronic resource].

Subject(s):

Online resources:

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In: Assiut Veterinary Medical Journal 2014.v.60(142)Summary: The present study investigated the influence of cumulus cells on vitrification of canine germinal vesicle stage oocytes, and assessed the ability of vitrified -warmed oocytes for further maturation in vitro. Selected cumulus-oocyte complexes (COCs) or cumulus-free oocytes or partially denuded were vitrified in DAP 213 solution. The vitrified-warmed oocytes were observed morphologically, and the plasma integrity was assessed. The viable oocytes in each group were cultured in TCM-199 for 72 h at 38.5 oc in 5% C02, 5% 02 and 90% N2. All media were supplemented with 10% ofFCS, 50 ng/mL of EGF, 2 Ug/mL of estradiol-17?, 0.1 IU/mL ofhCG, 0.1 IU/mL of FSH, 0.25 mM of pyruvic acid, 100 UM of ?-mercaptoethanol, and antibiotics. The viability of vitrified-warmed intact oocytes (52.8%) was higher than denuded oocytes (33.9%, P < 0.05), however, there is no differences between the partially denuded oocytes (43.9%) and denuded oocytes (33.9%, P > 0.05).

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The present study investigated the influence of cumulus cells on vitrification of canine germinal vesicle stage oocytes, and assessed the ability of vitrified -warmed oocytes for further maturation in vitro. Selected cumulus-oocyte complexes (COCs) or cumulus-free oocytes or partially denuded were vitrified in DAP 213 solution. The vitrified-warmed oocytes were observed morphologically, and the plasma integrity was assessed. The viable oocytes in each group were cultured in TCM-199 for 72 h at 38.5 oc in 5% C02, 5% 02 and 90% N2. All media were supplemented with 10% ofFCS, 50 ng/mL of EGF, 2 Ug/mL of estradiol-17?, 0.1 IU/mL ofhCG, 0.1 IU/mL of FSH, 0.25 mM of pyruvic acid, 100 UM of ?-mercaptoethanol, and antibiotics. The viability of vitrified-warmed intact oocytes (52.8%) was higher than denuded oocytes (33.9%, P < 0.05), however, there is no differences between the partially denuded oocytes (43.9%) and denuded oocytes (33.9%, P > 0.05).

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