Preparation of new mycoplasma gallisepticum live vaccines and their evaluation in chickens [electronic resource].

Includes references.

This study comprised the preparation of local live Mycoplasma gallisepticum (MG) vaccines (ts-II and 6/85 strains), and their evaluation in comparison with F strain vaccine in chickens through laboratory experiment, using PCR and RAPD techniques for the detection of Mycoplasma vaccine strains. Eighty, day-old Mycoplasma free chicks (Avian 48 breed) were divided into eight groups, subjected to blood and tracheal swabs at the first, till sixth wcek postvaccination. Serum plate agglutination (SPA) test showed the highest positive MG antibodies in F strain vaccinated and the control positive groups. Followed by ts-II, 6/85 vaccinated and contact groups, and F strain contact group. Control positive group showed the highest geometric mean titcr (1054.32) by ELISA, followed by F strain vaccinated and its contact (932.03 and 818.81, respcctively). While ts-II and 6/85 vaccinated and contact groups were suspected (734.43, 703.04, 673.62 and 525.78, respectively). MG could bc isolated from 100% of the control positive and F strain vaccinated group and increased from 50-90% in thc F strain contact group. Isolation rate increased to 80% in ts-II vaccinated group, but it was only 50% in its contact one. In 6/85 vaccinated and contact groups,

Summary in Arabic.

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