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Effect Of Different Doses Of Cadmium Chloride On Interleukin-2, Lymphocyte Transformation And DNA Fragmentation In Adult Male Albino rats [electronic resource].

By:

EI-Hady, Kawther A

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Language: English Summary language: Arabic Description: p.89-100Other title:

تأثير جرعات مختلفة من كلوريد الكادميوم على الانترلوكين-2 ومعدل تكسير الحامض النووى الديوكسى lymphocyte transformation [Added title page title]

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Zagazig veterinary journal, 2010 v. 38 (5) [electronic resource].

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In: Zagazig Veterinary Journal 2010.v.38(5)Summary: Forty adult male albino rats were divided randomly into 4 equal groups (one control and three treated groups). The control group received only dis. water, while the treated groups received 5 ppm, 50 ppm and 100 ppm of cadmium chloride (CdC12) for consecutive 90 days in drinking water. Blood samples were collected just before scarification of the rats and serum was separated. The level of interleukin-2 in the serum of control and treated male albino rats was measured by ELISA. Lymphocyte transformation and DNA fragmentation assay in blood lymphocyte of rats were evaluated. At Day 90 of the experiment, rats were sacrificed by cervical dislocation and thymus was collected, weighted and stored in 10% neutral buffered formalin for histopathology. Data were analyzed by one-way analysis of variance using GraphPad Prism software.

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Forty adult male albino rats were divided randomly into 4 equal groups (one control and three treated groups). The control group received only dis. water, while the treated groups received 5 ppm, 50 ppm and 100 ppm of cadmium chloride (CdC12) for consecutive 90 days in drinking water. Blood samples were collected just before scarification of the rats and serum was separated. The level of interleukin-2 in the serum of control and treated male albino rats was measured by ELISA. Lymphocyte transformation and DNA fragmentation assay in blood lymphocyte of rats were evaluated. At Day 90 of the experiment, rats were sacrificed by cervical dislocation and thymus was collected, weighted and stored in 10% neutral buffered formalin for histopathology. Data were analyzed by one-way analysis of variance using GraphPad Prism software.