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Detection of lumpy skin disease virus antigen and DNA in formalin fixed paraffin embedded tissues from a natural outbreak in Egypt [electronic resource].

By:

Awadin, Walaa

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Language: English Summary language: Arabic Description: p.65-77Other title:

تحديد وجود أنتيجينات فيروس إلتهاب الجلد العقدى فى بعض أنسجة العجول المحفوظة فى الفورماليين [Added title page title]

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Mansoura Veterinary Medical Journal, 2010 v. 12 (2) [electronic resource].

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In: Mansoura Veterinary Medical Journal 2010.v.12(2)Summary: An epidemic of Lumpy skin disease (LSD) affecting 16 provinces in Egypt was reported during 2006, Formalin fixed tissues including biopsies and postmortem tissue samples were collected from calves showed typical clinical signs of LSD from a private dairy farm in Damietta province, Egypt a period from March to May, 2006, Formalin fixed paraffin embedded tissue samples (FFPET) were assessed using histopathology as the skin lesions were classified into either acute or subacute-chronic. Both LSD viral DNA detected by polymerase chain reaction (PCR) of extracted DNA and LSD antigen detected by immunohistochemistry (IHC) using a capripOXvirus specific monoclonal antibody were observed in the acute skin lesions and in some subacutechronic skin lesions.

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An epidemic of Lumpy skin disease (LSD) affecting 16 provinces in Egypt was reported during 2006, Formalin fixed tissues including biopsies and postmortem tissue samples were collected from calves showed typical clinical signs of LSD from a private dairy farm in Damietta province, Egypt a period from March to May, 2006, Formalin fixed paraffin embedded tissue samples (FFPET) were assessed using histopathology as the skin lesions were classified into either acute or subacute-chronic. Both LSD viral DNA detected by polymerase chain reaction (PCR) of extracted DNA and LSD antigen detected by immunohistochemistry (IHC) using a capripOXvirus specific monoclonal antibody were observed in the acute skin lesions and in some subacutechronic skin lesions.

Summary in Arabic.

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