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Use of tissue print-immunoblotting for detection of barley yellow dwarf luteovirus [electronic resource].

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Salama, M. I

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Language: English Summary language: Arabic Description: p.47-55Other title:

استخدام بصمة النسيج-النقل المناعى للكشف عن فيروس التقزم الأصفر فى الشعير [Added title page title]

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Annals of agricultural science, 2003 v. 48 (1) [electronic resource].

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In: Annals of Agricultural Science 2003.v.48(1)Summary: Direct tissue blot inununoblotting assay (TBIA) on nitrocellulose membrane was successfully used for the detection of barley yellow dwarf luteovirus (BYDV) in artificially virus-infected stems of barley plants. In this study, the virus antigens on the blots were detected by means of goat antirabbit (GAR) labeled with alkaline phosphatase (AP), as a clear difference between the virus-infected sample (a purplish-blue color) and non-infected control was appeared.

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Direct tissue blot inununoblotting assay (TBIA) on nitrocellulose membrane was successfully used for the detection of barley yellow dwarf luteovirus (BYDV) in artificially virus-infected stems of barley plants. In this study, the virus antigens on the blots were detected by means of goat antirabbit (GAR) labeled with alkaline phosphatase (AP), as a clear difference between the virus-infected sample (a purplish-blue color) and non-infected control was appeared.

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