Comparison between different ELISA procedures for detection of some Vicia faba viruses [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.57-67Other title:
  • مقارنة بين طرق مختلفة من الأليزا للكشف عن بعض الفيروسات التى تصيب نبات الفول البلدى [Added title page title]
Uniform titles:
  • Annals of agricultural science, 2003 v. 48 (1) [electronic resource].
Subject(s): Online resources: In: Annals of Agricultural Science 2003.v.48(1)Summary: In this study three different procedures of enzyme-liked imrnunosorbent assay (ELISA) were evaluated for detecting the most important viruses affecting faba bean. These viruses are Faba bean necrotic yellows virus (FBNYV), Bean yellow mosaic virus (BYMV), Broad bean mottle virus (BBMV), and Broad bean stain virus (BBSV). In each test six dilutions, i.e., 1/20, 1/100, 1/500, 1/1000, 1/2500 and 1/5000 were used. Among the results, the enzyme amplification-ELISA (EAELISA) was the most sensitive, where the BBMV, BYMV, BBSV and FBNYV were detected in sap dilution of 1/2500, 1/2500, 1/1000 and 1/1000, respectively.
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In this study three different procedures of enzyme-liked imrnunosorbent assay (ELISA) were evaluated for detecting the most important viruses affecting faba bean. These viruses are Faba bean necrotic yellows virus (FBNYV), Bean yellow mosaic virus (BYMV), Broad bean mottle virus (BBMV), and Broad bean stain virus (BBSV). In each test six dilutions, i.e., 1/20, 1/100, 1/500, 1/1000, 1/2500 and 1/5000 were used. Among the results, the enzyme amplification-ELISA (EAELISA) was the most sensitive, where the BBMV, BYMV, BBSV and FBNYV were detected in sap dilution of 1/2500, 1/2500, 1/1000 and 1/1000, respectively.

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