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Studying the pathogenicity and RAPD-PCR analysis of different Escherichia coli serotypes isolated from broilers and layer chickens [electronic resource].

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Taha, M

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Language: English Summary language: Arabic Description: p.224-236Other title:

دراسة مدى ضراوة وتحليل البلمرة المتسلسل العشوائى لحامض الدى إن أية الغير متشابه للإنماط السيرولوجية المختلفة من الميكروب القولونى المعزول من قطعان البدارى والدجاج البياض [Added title page title]

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Assiut veterinary medical journal, 2002 v. 46 (92) [electronic resource].

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In: Assiut Veterinary Medical Journal 2002.v.46(92)Summary: In the present study successful isolation of Escherichia coli (E. coli) was carried out from freshly dead and diseased birds. The isolates were biochemically and serologically identified using 0- antigen including eight different serotypes (02, 08, 011, 022, 055, 087, 0127 and 0 157) beside six untypable strains. Pathogenicity of all the isolated strains was tested in 7 days - old chicks by subcutaneous inoculation of 108 CFU/ml. Genetic variation of 30 isolates was analysed using the random amplified polmorphic DNA (RAPD) technique. A dendograph generated using a random primer of arbitrary sequence demonstrated 6 major clusters. Each cluster encompassed small clusters corresponding to different serotypes including the untypable strains.

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In the present study successful isolation of Escherichia coli (E. coli) was carried out from freshly dead and diseased birds. The isolates were biochemically and serologically identified using 0- antigen including eight different serotypes (02, 08, 011, 022, 055, 087, 0127 and 0 157) beside six untypable strains. Pathogenicity of all the isolated strains was tested in 7 days - old chicks by subcutaneous inoculation of 108 CFU/ml. Genetic variation of 30 isolates was analysed using the random amplified polmorphic DNA (RAPD) technique. A dendograph generated using a random primer of arbitrary sequence demonstrated 6 major clusters. Each cluster encompassed small clusters corresponding to different serotypes including the untypable strains.

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