Identification and molecular studies on some halotolerant streptomycetes isolated from Sinai sandy soil [electronic resource].

Includes references.

Five streptomycete isolates obtained from Sinai sandy soil in Egypt, having the ability to tolerate 9-12% NaCl in the growth medium, were identified. Results showed that two out of them are belonging to the violet series (Si-1 and Si-9) and the other three isolates are belonging to the red series (Si-4, Si-6 and Si-11). Results of the cultural, morphological and physiological characteristics showed that Streptomyces isolate Si-1 a novel violet species. Four isolates were identified as S. tuirus Si-4, S. lateritius Si-6, S. mauvecolor Si-9 and S. melanogenes Si-11. Data of RAPD-PCR identification revealed that Streptomyces strain Si-1 had dissimilarities ranging from 60.9 to 65.1% with the red series isolates and 58.5% with the Si-9 violet series isolate. The dissimilarities between the three red series isolates were 33.3, 46.9 and 50.3%. Unique and specific PCR fragments were obtained for each strain; therefore, the results of this work paid an attention to encourage the use of RAPD-PCR technique as a new molecular tool for identification of actinomycetes.

Summary in Arabic.

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