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Peroxidase isozyme (s) as a biochemical marker for chocolate spot disease resistance gene (s) in faba bean (Vicia faba L.) [electronic resource].

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Abou Shousha, A.A

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Language: English Summary language: Arabic Description: p.97-113Other title:

استخدام المشابهات الإنزيمية للبيروكسيديز كدلائل بيوكيميائية لجينات المقاومة لمرض التبقع الشيكولاتي فى الفول البلدي [Added title page title]

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Journal of agricultural research Tanta University, 2001 v.27 (1) [electronic resource].

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In: Journal of Agricultural Research Tanta Univeristy 2001.v.27(1)Summary: The present study was conducted to test if the peroxidase isozyme could be used as a biochemical genetic marker associated with the chocolate spot disease (Botrytis fabae) resistance gene(s) in faba bean (Vicia faba). Peroxidase isozymes were extracted from the leaves of two faba bean cultivars showing different response to the disease. i.e. Giza 402 (susceptible) and Giza 461 (resistant), and their F1, F2 crosses in two directions. The test was made under artificial infection with two isolates of the causal pathogen in the greenhouse. The results showed differences in number, migration and density of bands between the two cultivaied varieties and their F1 plants Also. the artificial infection with the pathogen induced an additional band with a high molecular weight than those of the control (uninfected). parents and F1 plants.

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The present study was conducted to test if the peroxidase isozyme could be used as a biochemical genetic marker associated with the chocolate spot disease (Botrytis fabae) resistance gene(s) in faba bean (Vicia faba). Peroxidase isozymes were extracted from the leaves of two faba bean cultivars showing different response to the disease. i.e. Giza 402 (susceptible) and Giza 461 (resistant), and their F1, F2 crosses in two directions. The test was made under artificial infection with two isolates of the causal pathogen in the greenhouse. The results showed differences in number, migration and density of bands between the two cultivaied varieties and their F1 plants Also. the artificial infection with the pathogen induced an additional band with a high molecular weight than those of the control (uninfected). parents and F1 plants.

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