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Assessment of genetic diversity for domesticated and wild wheat [electronic resource].

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Khaled, A. E

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Language: English Summary language: Arabic Description: p.508-522Other title:

تقييم التنوع الوراثي للأقماح المنزرعة والبرية [Added title page title]

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Journal of the advances in agricultural researches, 2013 v. 18 (3) [electronic resource].

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In: Journal of the Advances in Agricultural Researches 2013.v.18(3)Summary: Diversity of four domesticated wheat cultivars or varieties (Sids 1, Sakha 93, Giza 168 and Gemmeiza 10) and two wild wheat (Aegilops ventricosa and Aegilops Kotchyi) were analyzed by morphological, biochemical and molecular analysis. Five morphological characteristics i.e. Heading to date (days), stem number/plant, number of spike/plant, gram number/spike and 1O00 grains weight (g) were calculated to show the difference among wheat cultivars and their relatives Aegilops species. High significant variations were observed among the wild and domesticated wheat cultivars. The four domesticated wheat cultivars were earlier in heading to date compared with the wild species with range 20 to 31 days in average. Biochemical analysis for peroxidase isozymes profile exhibited three marker bands (PxA,, PxC1 and PxC3 ) for the wild type cultivars, also Ae. ventricose expressed unique marker band at Px5c locus. Fourteen (10 mer) RAPD-PCR were used to detect the genetic diversity. In Total of 550 amplified fragments, 51 DNA specific markers were detected. The number of reproducible bands/primer varied between 18 for primer OPC-12 and 56 for primer OPH-11 with a total of 550 bands. The largest number of these markers was specific for wild wheat, A e. ventricoas and Ae. Kotchyi (20 and 12 markers, in respect).

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Diversity of four domesticated wheat cultivars or varieties (Sids 1, Sakha 93, Giza 168 and Gemmeiza 10) and two wild wheat (Aegilops ventricosa and Aegilops Kotchyi) were analyzed by morphological, biochemical and molecular analysis. Five morphological characteristics i.e. Heading to date (days), stem number/plant, number of spike/plant, gram number/spike and 1O00 grains weight (g) were calculated to show the difference among wheat cultivars and their relatives Aegilops species. High significant variations were observed among the wild and domesticated wheat cultivars. The four domesticated wheat cultivars were earlier in heading to date compared with the wild species with range 20 to 31 days in average. Biochemical analysis for peroxidase isozymes profile exhibited three marker bands (PxA,, PxC1 and PxC3 ) for the wild type cultivars, also Ae. ventricose expressed unique marker band at Px5c locus. Fourteen (10 mer) RAPD-PCR were used to detect the genetic diversity. In Total of 550 amplified fragments, 51 DNA specific markers were detected. The number of reproducible bands/primer varied between 18 for primer OPC-12 and 56 for primer OPH-11 with a total of 550 bands. The largest number of these markers was specific for wild wheat, A e. ventricoas and Ae. Kotchyi (20 and 12 markers, in respect).

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