Production of specific antiserum for bean yellow mosaic virus and broad bean stain virus [electronic resource].

Includes references.

Bean yellow mosaic Potyvirus (BYMV) and Broad bean stain Comovirus (BBSV) were isolated from El-Sharkia and Bani-Seuf governorates and purified using differential centrifugation. Ulteraviolate absorbance of purified viruses were typical for nucleoprotein with minimum at 243, 244 nm and maximum at 260, 260 for BYMV and BBSV respectively. The ratio of A 260 /A 280 and A max/min were 1.33 and 1.llfor BYMV respectively and A 260 /A 280 and A max/min ratio were 1.68 and 1.23 for BBSV respectively. The yield of purified virus was 5.55 mg/100 gm infected leaves and 4.78 mg/kg infected leaves for BYMV and BBSV respectively. Production of specific antisera were obtained by rabbit immunization with different amount of antigen (1,2 and 3 mg/ml) for BYMV and (1 and 2 mg/ml) for BBSV using three different methods of injection. The titer of the prepared antisera as determined by indirect ELISA at dilution of tissue 1/5 were 1/2048 and 1/1024 for BYMV and BBSV respectively.

Summary in Arabic.

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