Prevalence of Listeria monocytogenes in raw and cooked poultry with rapid confirmation by multiplex PCR [electronic resource].

Includes references.

The incidence of Listeria spp, and Listeria monocytogemes in raw, grilled and fried poultry was determined, 40 samples of each product wich were collected from different localities in Assiut City, The obtained results pointed out that Listeria spp. could be isolated from 50, 15 and 7.5% of the examined raw, grilled and fried poultry respectively, while(20)of Listeria momocytogenes was detected in 14 (35%) of raw poultry, 5 (12.5%) of grilled samples and I (2.5%) of fried samples. Strains of Listria monocylogenes was characterized by several biochemical tests and confirmed by polymerase chain reaction (PCR). Results showed that Listeria moncytogenes was confirmed by PCR in 6 isolates of raw poultry and one from each of fried and grilled samples. This study revealed that presence of Listeria monocytogenes in both grilled and fried poultry is an important public risk because of the great demand for daily consumption of these products.

Summary in Arabic.