Velocity of specific selective enzymes in mango (Mangifera indica L.) treated with y-rays [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.23-41Other title:
  • نشاط بعض الإنزيمات المختارة في المانجو المعامل بأشعة جاما [Added title page title]
Uniform titles:
  • Alexandria journal of food science and technology, 2004 v.1 (1) [electronic resource].
Subject(s): Online resources: In: Alexandria Journal of Food Science and technology 2004.v.1(1)Summary: The problem at hand is dealing with to what extend velocities of polyphenol oxidase (PPO), peroxidase (POD) and pectinmethylesterase (P:rvtE) are affected by irradiation? To reach such target, the investigated man~o fruits were irradiated with different doses of y- rays namely .500, 1000, 1500,2500 and 5000 Gy produced from Co o. Velocity reaction of the aforementioned enzymes in relation to different substrate and enzyme concentrations were studied. Kinetic properties in tenus of affinity, maximum velocity, activity coefficient, and slope of reaction were calculated through a specific computer program namely "Enzyme kinetics". Changes in the velocity patterns of the investigated enzymes as affected by storage for three weeks at 20±2°C were also studied. On the basis ofthe obtained results, it was found lhat a dose 0[2500 Gy could be recommended Lo reach higher inhibition level of the three tested enzymes, a pattern which improves the quality and extends shelf-life of mango fruit. Key Words: Mango, irradiation, PPO, POD and PME enzymes.
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The problem at hand is dealing with to what extend velocities of polyphenol oxidase (PPO), peroxidase (POD) and pectinmethylesterase (P:rvtE) are affected by irradiation? To reach such target, the investigated man~o fruits were irradiated with different doses of y- rays namely .500, 1000, 1500,2500 and 5000 Gy produced from Co o. Velocity reaction of the aforementioned enzymes in relation to different substrate and enzyme concentrations were studied. Kinetic properties in tenus of affinity, maximum velocity, activity coefficient, and slope of reaction were calculated through a specific computer program namely "Enzyme kinetics". Changes in the velocity patterns of the investigated enzymes as affected by storage for three weeks at 20±2°C were also studied. On the basis ofthe obtained results, it was found lhat a dose 0[2500 Gy could be recommended Lo reach higher inhibition level of the three tested enzymes, a pattern which improves the quality and extends shelf-life of mango fruit. Key Words: Mango, irradiation, PPO, POD and PME enzymes.

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