Epidemiological studies of T. evansi in camels in Saudi Arabia using real time polymerase chain reaction technique [electronic resource].

Includes references.

Trypanosomasis (surra) still a serious problem in camel husbandry world widly. It causes considerable economic losses in many camel-rearing regions. A little date for Real Time polymerase chain reaction (PCR) for rapid detection Trypanosoma evansi of blood from naturally infected camels were puplished. In the present study 242 blood samples from clinically suspected camels to surra from four province of Saudia (AI¬Riyadh, AI- Qassim, Alharig, and AI-Dawadmi) were collected and used for further examination. Real Time PCR-based assay for blood samples were conducted in direct detection of T evans; DNA from the collected blood samples using specific primer set (TR3/TR4) derived from nuclear repetitive gene of Tevansi and SYBER Green! fluorescent dye have been used. The melting peak chart of the positive samples showed one single peak at an average Tm of 85.0°C. The performed real time PCR technique was found accurate method for diagnosis of trypanosome.

Summary in Arabic.