Honeybee collected pollen as Antibiotics to control American foul brood disease, at Giza governorate [electronic resource]

Includes bibliographic reference

The aim of this study was to determine the phenolic
compounds and antibacterial activity components of pollen
grains, obtained from different concentrations extracts of
ethanol and water for controlling American foulbrood (AFB)
disease. In this study four Egyptian bee pollen grains types were
collected from pollen basket, namely Broad bean ( Vicia faba), date
palm (Phoenix dactylifera), clover ( Trifolium alexandrinum) and
maize (Zea mays), during spring. Identification of Phenolic
composition in pollen grains samples were investigated by high
performance liquid chromatography (HPLC) instrument. Two pollen
extracts, using ethanol and water by three concentrations 5, 10
and 20% agent's Paenibacillus larvae subspecies larvae, formerly
Bacillus larvae and it's potential for the control of American
foulbrood disease (AFB) in vitro. Generally the concentration of 5%
gave no effect neither pollen ethanol or aqueous extracts. But
pollen grain water extracts gave inhibition zone diameter with
different pollen grain of types (10%) and (20%) concentrations.
The water extract of date palm pollen grains recorded a highly
effect of inhibition zone which were 21.30& 28.65mm with
10&20% concentrations respectively. This study considers a
primary step for the pollen grains on P. larvae spores in vitro.
These products showed efforts no toxicity effects on different stage
of honeybee. Suggesting them as perspective, low cost and
consumer-acceptable agent for control of AFB disease in honeybee
colonies.

Summary in Arabic