Purification, serology and molecular detection of Egyptian isolates of banana bunchy top babuvirus and faba bean necrotic yellows nanovirus [electronic resource].

Includes references.

Two isolates of faba bean necrotic yellows nanovints (FBNYV) and banana bunchy top babuvints (BBTV) were purified. Cellulase added to the extraction buffers (EB) enhanced extraction of both vintses. An acidic pH was used for FBNYV extraction. Several trials for purification of BBTV indicated that alkaline condition surpassed acidic condition in virus extraction. Polyethylene glycol and NaCI were used to concentrate both viruses. Electroeilition coupled with freezing and thawing were employed in further purification steps. Purified FBNYV yielded 677 ~g/ g tissue and with A 260/280 ratio of 1.43. Purified BBTV yielded 12.9 ~g /g tissue and with A 260/280 ratio of 1.3. Both viruses had a single-capsid protein of 20 kDa.

Summary in Arabic.

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