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Association between A31GSNP of the myostatin gene and serum levels of ALP,GOT, P and Ca in Egyptian buffalo (bubalus bubalis) [electronic resource].

By:

Saad, Michel Fahmy

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Description: p.111-126Uniform titles:

kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].

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In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: Myostatin, a member of the transforming growth factor B (TGF-B) superfamily, is a negative regulator of skeletal muscle growth in mammals. The loss or inhibition of myostatin signaling dramatically increases muscle mass. This study is aimed to detect single nucleotide polymorphisms (SNPs) in the myostatin (Mstn) gene and to associate measure their effect on mineral (Ca and P) and enzymatic (GOT and ALP) serum levels in high and low meat producing Egyptian buffaloes. Mstn gene was amplified by PCR and subsequently, subjected to sequence analysis to identify different allelic patterns. The nucleotide sequence analyses revealed presence of a novel A31GSNP in Mstnl locus at nucleotide number 50 before exon2. This SNP was synonymous as it is located in the non coding sequence and so no amino acid was changed. However, the sequences of the other three loci showed 100% identity among the Egyptian buffaloes and as compare to the published sequences of buffalo (GenBank accession number, DQ091762).

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Myostatin, a member of the transforming growth factor B (TGF-B) superfamily, is a negative regulator of skeletal muscle growth in mammals. The loss or inhibition of myostatin signaling dramatically increases muscle mass. This study is aimed to detect single nucleotide polymorphisms (SNPs) in the myostatin (Mstn) gene and to associate measure their effect on mineral (Ca and P) and enzymatic (GOT and ALP) serum levels in high and low meat producing Egyptian buffaloes. Mstn gene was amplified by PCR and subsequently, subjected to sequence analysis to identify different allelic patterns. The nucleotide sequence analyses revealed presence of a novel A31GSNP in Mstnl locus at nucleotide number 50 before exon2. This SNP was synonymous as it is located in the non coding sequence and so no amino acid was changed. However, the sequences of the other three loci showed 100% identity among the Egyptian buffaloes and as compare to the published sequences of buffalo (GenBank accession number, DQ091762).

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