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Comparison of the efficiency of some novel maize promoters in monocot and dicot plants [electronic resource]

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Assem, Shireen K. Assem

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Language: English Summary language: Arabic Description: p.57-66Other title:

مقارنة كفاءة بعض المحفزات الجديدة المعزولة من الذرة الشامية فى نباتات أحادية ؟ ثنائية الفلقة [Added title page title]

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Arab journal of biotechnology, 2002 v. 5 (1) [electronic resource]:

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In: Arab Journal of Biotechnology 2002.v.5(1)Summary: Three novel maize promoters (Gos-2, Enolase and Actin-2) were evaluated for their efficiency in driving the GUS gene expression in plant tissues. Three plasmids were constructed to contain the GUS gene in combination with the three promoters and another three plasmids were constructed to contain the GUS gene in combination with the three promoters and the INTIUBIZM intron. The six plasmids were employed in the transformation of maize immature embryos (as a monocot system) and tomato leaflets (as a dicot system). The transformation was conducted using the biolistic particle delivery system. Our results revealed the efficiency of the newly isolated maize promoters in both monocots and dicots. However, the level of activity of the different promoters was variable in both systems.

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Three novel maize promoters (Gos-2, Enolase and Actin-2) were evaluated for their efficiency in driving the GUS gene expression in plant tissues. Three plasmids were constructed to contain the GUS gene in combination with the three promoters and another three plasmids were constructed to contain the GUS gene in combination with the three promoters and the INTIUBIZM intron. The six plasmids were employed in the transformation of maize immature embryos (as a monocot system) and tomato leaflets (as a dicot system). The transformation was conducted using the biolistic particle delivery system. Our results revealed the efficiency of the newly isolated maize promoters in both monocots and dicots. However, the level of activity of the different promoters was variable in both systems.

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