Cryopreservation of in vitro established shoot tip explants of date palm cv. Zaghlool [electronic resource].

Includes reference.

Cryoprotection procedures for in vitro established shoot tip explants of Date palm cv. Zaghlool prior to Cryoprservation process in liquid nitrogen. were investigated in this study. This work aimed to develop simple cryoprotection procedure that can be handled easily. The first cryoprolection procedure was to study the effects of different sucrose concentrations (0.1, 0.3, 0.5, and 0.7Mol) in preculturing medium and the incubationtion under 5'C or 27C for four weeks. The second cryoprotection procedure was to study the effects of the same previous sucrose concentrations in preculturing medium and the incubation under 27'C for four weeks followed by desiccation procedure for two hours in an open petri dish exposed to continual current of sterile air in the laminar airflow cabinet

Summary in Arabic.

1