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PCR cloning of polyhydroxybutyrate synthase gene (PhbC) from Aeromonas hydrophila [electronic resource].

By:

Enan, M. R

Contributor(s):

Bashandy, S. A

Language: English Summary language: Arabic Description: P. 157-164Other title:

إستنساخ جين السينسيز المسؤل تخليق مركب البولى هيدروكسى بيوتيرات من بكتيريا الإيرومونس هيدروفيلا بإستخدام التفاعل التسلسلى للبوليميراز [Added title page title]

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Arab journal of biotechnology, 2004 v. 7 (2) [electronic resource]:

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In: Arab Journal of Biotechnology 2004.v.7(2)Summary: Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by a high production cost. A Polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate. PCR strategy using PHB primers resulted in the amplification of DNA fragments with the expected size from all isolated bacteria. PHB synthase gene was cloned directly from Aeromonas hydrophila genome for the first time from these bacteria. The cloned fragment was named phb CAh. This fragment was partially sequenced and open reading frame was found representing the PHB synthase gene. The nucleotide sequence of phbCAh gene exhibits similarity to Caulobacter crescentus phbC (40.3%), Pseudomonas oleovorans phbC (34.3%), and Rhodobacter sphaeroides phbC (40.6 %).

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Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by a high production cost. A Polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate. PCR strategy using PHB primers resulted in the amplification of DNA fragments with the expected size from all isolated bacteria. PHB synthase gene was cloned directly from Aeromonas hydrophila genome for the first time from these bacteria. The cloned fragment was named phb CAh. This fragment was partially sequenced and open reading frame was found representing the PHB synthase gene. The nucleotide sequence of phbCAh gene exhibits similarity to Caulobacter crescentus phbC (40.3%), Pseudomonas oleovorans phbC (34.3%), and Rhodobacter sphaeroides phbC (40.6 %).

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