Producing super male (ZZ) and using RAPD-PCR technique for sex differentiation in blue tilapia (Oreochromis aureus) [electronic resource].

Includes references.

A study was made to identify sex differentiation in parents and fries of Oreochromis aureus using RAPD- PCR and squash techniques. Fishes were collected from the experiment shared in program for producing "super males ZZ" in Egypt. The trial of sex reversal for feminization of sexually undifferentiated progeny was conducted using 17-B ethynylestradiol as a feminizing stimulating agent. Oral administration of powdered feed containing 400 and 800 mg/kg were tested for 30 days in Central Laboratory for Aquaculture Research in Abassa (CLAR). Upon termination of the experiment, average weight and length have increased using high dose. In contrast, the survival rate was increased in low dose ( 400 mg/kg) than high dose (800 mg/kg). Its effect was highly detectable among fry that were orally fed on 800-mg/kg hormone-treated feeds for 30 days. The mean percentage of phenotypic females was 94% ± 2.7 in the second season, females generated progeny including mean percentage of phenotypic males was 69%±4.6 while female, inter sex and non differential percentages were 26% ±3.8, 3% ± 1.5 and 2%±0.7 respectively. the sex differentiated have been using applied squash method and RAPD-PCR , Squash method illustrated that from every 10 fishes, 9 individuals were converted into super male (ZZ) .

Summary in Arabic.

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