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Effects of genotypes, explants and media on potato callus induction and plantlets regeneration [electronic resource].

By:

Abou Shousha, A. A

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Language: English Summary language: Arabic Description: p.84-96Uniform titles:

Journal of agricultural research Tanta University, 2001 v.27 (1) [electronic resource].

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In: Journal of Agricultural Research Tanta Univeristy 2001.v.27(1)Summary: This studv was conducted to evaluate the genetic diversity of some tetraploid potatos (Solanum tuberosum) on callus induction, callus weight and plant regeneration. Five genotypes (cultivars) i e, Arinda, Diamont, King Edward, Nicola and HPS 1/67 were used as a sourse of three explants (stem segments , root-tips and leaf discs) Four media were used for callus induction using MS medium supplemented with different growth regulators, whereas four regeneration media were adapted to investigate the ability of producing regenerates from callus. • Results showed that the potato variety Nicola gave the highest vigorous callus (100%) than other cultivars on CM4 using stem segments. The same cultivar recorded the highest callus fresh weight (790.0 mg) using stem explants on CM2 . On the other hand, Diamont gave the highest regeneration percentage (67.1 %) when callai were cultured on RM1 compared with King Edward cultivar which yielded 13.5% on RM3.

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This studv was conducted to evaluate the genetic diversity of some tetraploid potatos (Solanum tuberosum) on callus induction, callus weight and plant regeneration. Five genotypes (cultivars) i e, Arinda, Diamont, King Edward, Nicola and HPS 1/67 were used as a sourse of three explants (stem segments , root-tips and leaf discs) Four media were used for callus induction using MS medium supplemented with different growth regulators, whereas four regeneration media were adapted to investigate the ability of producing regenerates from callus. • Results showed that the potato variety Nicola gave the highest vigorous callus (100%) than other cultivars on CM4 using stem segments. The same cultivar recorded the highest callus fresh weight (790.0 mg) using stem explants on CM2 . On the other hand, Diamont gave the highest regeneration percentage (67.1 %) when callai were cultured on RM1 compared with King Edward cultivar which yielded 13.5% on RM3.

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