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Evaluation of mycobacterium bovis sarkosyl extract for diagnosis of bovine tuberculosis in buffaloes [electronic resource].

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Nasr, E. A

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Language: English Summary language: Arabic Description: p.579 - 586Other title:

تقييم انتيجين محضر بالساركوسيل من الميكوبكتيريم بوفيس لتشخيص السل البقري فى الجاموس [Added title page title]

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Beni-Suef veterinary medical journal, 2001 v. 11 (2B) [electronic resource].

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In: Beni-Suef Veterinary Medical Journal 2001.v. 11(2B)Summary: A modified indirect ELISA was conducted on sera from 50 tuberculin positive and 38 tuberculin negative buffaloes, using human and bovine PPD tuberculins in addition to sarkosyl extract from Mycobacterium bovis. The results revealed that the sarkosyl extract gave the best discrimination between tuberculous and non- tuberculous animals, as higher sensitivity, specificity and efficiency of prediction than either types of PPD was recorded. The probability of misclassification cleared that only 18% of tested animals would be misclassified by the sarkosyl extract, in comparison to 28% and 25.7% for human and bovine PPDs respectively. The use of serological assays, using semi purified antigens, such as sarkosyl extract, as a complement to intradermal tuberculin skin test, would greatly increase the efficiency of eradication programs.

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A modified indirect ELISA was conducted on sera from 50 tuberculin positive and 38 tuberculin negative buffaloes, using human and bovine PPD tuberculins in addition to sarkosyl extract from Mycobacterium bovis. The results revealed that the sarkosyl extract gave the best discrimination between tuberculous and non- tuberculous animals, as higher sensitivity, specificity and efficiency of prediction than either types of PPD was recorded. The probability of misclassification cleared that only 18% of tested animals would be misclassified by the sarkosyl extract, in comparison to 28% and 25.7% for human and bovine PPDs respectively. The use of serological assays, using semi purified antigens, such as sarkosyl extract, as a complement to intradermal tuberculin skin test, would greatly increase the efficiency of eradication programs.

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