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M9 protein as an ELISA antigen for evaluation of immune response to Mycoplasma gallisepticum [electronic resource].

By:

Salama, S. S

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Language: English Summary language: Arabic Description: p.75-85Other title:

استخدام البروتين M9 كأنتجن للإليزا لتقييم الإستجابة المناعية لميكروب الميكوبلازما جاليسبتكم [Added title page title]

Uniform titles:

Zagazig veterinary journal, 2005 v. 33 (3) [electronic resource].

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In: Zagazig Veterinary Journal 2005.v.33(3)Summary: PCR amplification of M.gallisepticum genome using the M9 gene primers succeeded to generate of DNA fragment of about 1 kb Size. The results of PCR agreed with that obtained by biochemical and serological identification. Regarding the molecular cloning of tne PCR product using the specific primers of M9 gene, the PCR product ligated directly into the vector then transformed into the competent cells and the recombinant plasmids with the correct inserts were validated by many preparation. and restriction followed by restriction digestion.

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PCR amplification of M.gallisepticum genome using the M9 gene primers succeeded to generate of DNA fragment of about 1 kb Size. The results of PCR agreed with that obtained by biochemical and serological identification. Regarding the molecular cloning of tne PCR product using the specific primers of M9 gene, the PCR product ligated directly into the vector then transformed into the competent cells and the recombinant plasmids with the correct inserts were validated by many preparation. and restriction followed by restriction digestion.

Summary in Arabic.

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