Tissue culture and evaluation of some active constituents of Ruta graveolens L. [electronic resource]: I- Effect of plant growth regulators and explant type on growth of Ruta graveolens L. callus cultures

Includes references.

The effect of plant growth regulators and explant type on growth of Ruta graveolens L. callus cultures was studied. The explants used were stem, leaf, shoot tip and root. The most successful concentrations of plant growth regulators on four MS basal solid media were I (MS+ 1 mg/l 2,4-D), II (MS+1mg/12,4-D+ 1mg/l kinetin), III (MS+0.5mg/l 2,4-D+ 0.5 mg/l kinetin) and IV (MS+lmg/l 2,4-D+ 5mg/l kinetin). The highest survival values in callus formation were those of stem and root cultures on medium III. Also, leaf and shoot tip cultures on medium II showed higher survival percent age amongst their cultures. The morphological characters of callus cultures were described. The effect of plant growth regulators and explant type on growth dynamics of callus (fresh weight, dry weight, callus index and callus vigor) was studied. Stem cultures on media I II, shoot tip on media II, III and IV, root cultures on media II and IV produced significant increase in the previous values, while leaf cultures on medium III produced significantly increased fresh weight and callus index values although dry weight and callus vigor values showed insignificant differences between different leaf cultures.

Summary in Arabic.

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