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Identification of novel SNP in prolactin promoter and its association with milk traits in Behairy Egyptian water buffaloes [electronic resource].

By:

Zaki, Abeer Gamal

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Description: p.247-259Uniform titles:

kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].

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In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: Prolactin hormone (PRL) plays a crucial role in the initiation and maintenance of lactation in the animals. It is a potenial quantitative trait locus and genetic marker of production traits in dairy cattle. However, no SNP was detected and no association studies was conducted on PRL gene in Egyptian buffalo up till now. Therefore, the objective of the present study was planned to detect PRL gene polymorphisms in Behairy Egyptian water buffalo APCR product of 245 bp, including part of the prommoter of PRL was amplified using PCR and subsequently SSCP and nucleotide sequencing were conducted to identify its different allelic patterns in Behairy Egyptian water buffaloes.

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Prolactin hormone (PRL) plays a crucial role in the initiation and maintenance of lactation in the animals. It is a potenial quantitative trait locus and genetic marker of production traits in dairy cattle. However, no SNP was detected and no association studies was conducted on PRL gene in Egyptian buffalo up till now. Therefore, the objective of the present study was planned to detect PRL gene polymorphisms in Behairy Egyptian water buffalo APCR product of 245 bp, including part of the prommoter of PRL was amplified using PCR and subsequently SSCP and nucleotide sequencing were conducted to identify its different allelic patterns in Behairy Egyptian water buffaloes.

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