Antibiogram and characterization of some plasmid mediated genes of salmonella species isolated from pigeon [electronic resource].

By: Contributor(s): Description: p.287-311Uniform titles:
  • kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].
Subject(s): Online resources: In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: To determine sensitivity of isolated Salmonella species to various antimicrobial agents and molecular characterization of some common genes responsible for antibiotic resistance phenotypes. In this study, bacteriological isolation of Salmonella species isolated from 400 samples which were collected from squabs and adult pigeon and their surroundings. Bacterial isolates were tested for their susceptibility to 17 different antimicrobial discs as mentioned before by the disc diffusion method. Using PCR for screening of antimicrobial resistance genes, the bacterial isolates were tested for OX4, SHV,TEM, CTX-M and CMY ?-lactamase-encoding genes by PCR using universal primers for the OXA, SHV, TEM, CTX-M and CMY families. Prevalence of Salmonella in slaughtered squabs liver ,intestine and intestinal lymph nodes was 1.8%, 5.5% and 3.6% respectively ;and in adult pigeons 1%, 3% and 2%, respectively. Sensitivity of all Salmonella isolates were completely resistant to Streptomycin, Amoxicillin/clavulanic acid, Amoxicillin, Ampicillin and Ceftazidime.
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To determine sensitivity of isolated Salmonella species to various antimicrobial agents and molecular characterization of some common genes responsible for antibiotic resistance phenotypes. In this study, bacteriological isolation of Salmonella species isolated from 400 samples which were collected from squabs and adult pigeon and their surroundings. Bacterial isolates were tested for their susceptibility to 17 different antimicrobial discs as mentioned before by the disc diffusion method. Using PCR for screening of antimicrobial resistance genes, the bacterial isolates were tested for OX4, SHV,TEM, CTX-M and CMY ?-lactamase-encoding genes by PCR using universal primers for the OXA, SHV, TEM, CTX-M and CMY families. Prevalence of Salmonella in slaughtered squabs liver ,intestine and intestinal lymph nodes was 1.8%, 5.5% and 3.6% respectively ;and in adult pigeons 1%, 3% and 2%, respectively. Sensitivity of all Salmonella isolates were completely resistant to Streptomycin, Amoxicillin/clavulanic acid, Amoxicillin, Ampicillin and Ceftazidime.

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