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Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitrol [electronic resource].

By:

Amer, H. A

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Language: English Summary language: Arabic Description: p.89 -109Other title:

تأثير موسم التكاثر والقدرة الاسترويدوجينية للخلايا المحببة والسائل الجريبي علي انضاج بويضات الجمل معمليا.‪‪ [Added title page title]

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Mansoura veterinary medical journal, 2003 v.5(1) [electronic resource].

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In: Mansoura Veterinary Medical Journal 2003.v.5(1)Summary: The effect of ovarian status and steroidogenic potential of granulosa cells and/or follicular fluid on the maturation of camel oocytes in vitro dUring rating and nOn-rating seasons were studied. The camel ovaries were collected immediately after slaughter.The oocytes were aspirated from follicles with 8-20 mm diameter for culture in maturation medium (MM) under different co-culture conditions, MM+No GCs (No Granulosa Cells), MM+FGCs (Fresh Granulosa Cells), MM+PGCs (Pre-incubated Cranulosa Cells),MM+10%FF (Follicular FlUid) and MM+FGCs+10%FF. The obtained results revealed that, the distribution of small follicles on ovaries was significantly (p<0.01) higher than the medium and pre-ovulatory follicles.

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The effect of ovarian status and steroidogenic potential of granulosa cells and/or follicular fluid on the maturation of camel oocytes in vitro dUring rating and nOn-rating seasons were studied. The camel ovaries were collected immediately after slaughter.The oocytes were aspirated from follicles with 8-20 mm diameter for culture in maturation medium (MM) under different co-culture conditions, MM+No GCs (No Granulosa Cells), MM+FGCs (Fresh Granulosa Cells), MM+PGCs (Pre-incubated Cranulosa Cells),MM+10%FF (Follicular FlUid) and MM+FGCs+10%FF. The obtained results revealed that, the distribution of small follicles on ovaries was significantly (p<0.01) higher than the medium and pre-ovulatory follicles.

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