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Cytopathic genotype 2 bovine viral diarrhea virus in dromedary camels [electronic resource].

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Yousif, A. A

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Language: English Summary language: Arabic Description: P. 123-140Other title:

فيروس الإسهال الفيروسي فى الأبقار ذو النوع السيتوباثولوجى الجينى 2 فى الجمال ذات السنام الواحد.‪‪ [Added title page title]

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Arab journal of biotechnology, 2004 v. 7 (1) [electronic resource]:

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In: Arab Journal of Biotechnology 2004.v.7(1)Summary: Pestivirus infections in domesticated ruminant species and pigs are worldwide problems with severe economic impact. A wide range of wild ruminant species can also acquire pestivirus infections. We isolated several pestiviruses from Egyptian dromedary camels (Camelus dromedarius) in 1995. The camels from which these pestiviruses were isolated had signs of reproductive and congenital disease. The camel isolates were shown to experimentally infect cattle and goats. To genetically characterize these isolates, RNA extracts from two cytopathic Egyptian camel isolates, Giza4 and Giza7 were amplified by RT-PCR using a nested set of primers complementary to sequences in the g"S of the pestivirus genome. The nested PCR product was characteristic of a bovine viral diarrhea virus (BVDV) type 2 for isolate Giza7 and of BVDV type 1 for Giza4. Giza7 gns nucleotide sequences were 82-88 %, 68.7-72 %, 66.5-67.2%, 68 % and 64 % homologous with BVDV-2, BVDV-1, BDV, CSFV and' Reindeer-1 viruses, respectively.

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Pestivirus infections in domesticated ruminant species and pigs are worldwide problems with severe economic impact. A wide range of wild ruminant species can also acquire pestivirus infections. We isolated several pestiviruses from Egyptian dromedary camels (Camelus dromedarius) in 1995. The camels from which these pestiviruses were isolated had signs of reproductive and congenital disease. The camel isolates were shown to experimentally infect cattle and goats. To genetically characterize these isolates, RNA extracts from two cytopathic Egyptian camel isolates, Giza4 and Giza7 were amplified by RT-PCR using a nested set of primers complementary to sequences in the g"S of the pestivirus genome. The nested PCR product was characteristic of a bovine viral diarrhea virus (BVDV) type 2 for isolate Giza7 and of BVDV type 1 for Giza4. Giza7 gns nucleotide sequences were 82-88 %, 68.7-72 %, 66.5-67.2%, 68 % and 64 % homologous with BVDV-2, BVDV-1, BDV, CSFV and' Reindeer-1 viruses, respectively.

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