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Application of real time - PCR for identification of sheep poxvirus, camel poxvirus and contagious Ecthyma virus isolates [electronic resource].

By:

Abdel Baky, M. H

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Language: English Summary language: Arabic Description: p.37-46Other title:

تطبيق إستخدام إختبار التفاعل المتسلسل لإنزيم البلمرة (الوقت الحقيقى) فى تعريف فيروسات جدرى الأغنام وجدرى الإبل وإلتهاب الجلد البثرى الوبائى.‪ [Added title page title]

Uniform titles:

Veterinary medical journal, 2008 v.56 (2) [electronic resource].

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In: Veterinary Medical Journal 2008.v.56(2)Summary: Recently, attention has been directed toward the application of Real time - PCR assays as a rapid and accurate tools for identification of Capripox, Parapox and Orthopox viruses that cause devastating diseases in farm animals in the Kingdom of Saudi Arabia. SYBR Green ( Real time fi PCR assays with primer pairs; Capri - Ks.l of Capripoxviruses, 045 Orf of Parapoxviruses and Q Orf of Panparapoxviruses were adopted on a panel of Saudi field isolates and reference strains of sheep and goat poxviruses, camel poxvirus, reference strains of Lumpy skin disease and vaccinia viruses,and Saudi field isolates of contagious ecthyma (Orf) virus.

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Recently, attention has been directed toward the application of Real time - PCR assays as a rapid and accurate tools for identification of Capripox, Parapox and Orthopox viruses that cause devastating diseases in farm animals in the Kingdom of Saudi Arabia. SYBR Green ( Real time fi PCR assays with primer pairs; Capri - Ks.l of Capripoxviruses, 045 Orf of Parapoxviruses and Q Orf of Panparapoxviruses were adopted on a panel of Saudi field isolates and reference strains of sheep and goat poxviruses, camel poxvirus, reference strains of Lumpy skin disease and vaccinia viruses,and Saudi field isolates of contagious ecthyma (Orf) virus.

Summary in arabic.

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