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Serological studies on pseudomonas Syringae Pv. Syringae the leaf spot disease pathogen of cane-apple (Arbutus Pavarii Pampanini) in Jabal Al-Akhdar Area- Libya [electronic resource].

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Mohamed, A.M.A

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Language: English Summary language: Arabic Description: p.95-105Other title:

دراسة مصلية للبكتريا Pseudomonas syringae pv. syringae المسببة لمرض تبقع نبات الشمارى بمنطقة الجبل الاخضر [Added title page title]

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Journal of the advances in agricultural researches, 2008 v. 13 (1) [electronic resource].

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In: Journal of the Advances in Agricultural Researches 2008.v.13(1)Summary: Polyclonal antiserum was produced against Pseudomonas syringae pv. syringae the causal agent of bacterial leaf spot on Cane-Apple (Arbutus pavarii Pampanini) in Jabal Al-Akhdar area- Libya. Results of the slide agglutination and Ouchterlony gel double diffusion tests showed positive reactions between bacterial isolate and its homologous antiserum. Regarding collecting dates of antiserum, indirect ELISA revealed that antiserum of the first collecting date (after 2 days of the last injection) was the best comparing with those of second and the third dates (7 and 14 days respectively) .Titre of the obtained antiserum was 1:10.24X104 as determined by indirect ELISA. Indirect ELISA revealed the efficiency of this antiserum for comparing among the different bacterial isolates.

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Polyclonal antiserum was produced against Pseudomonas syringae pv. syringae the causal agent of bacterial leaf spot on Cane-Apple (Arbutus pavarii Pampanini) in Jabal Al-Akhdar area- Libya. Results of the slide agglutination and Ouchterlony gel double diffusion tests showed positive reactions between bacterial isolate and its homologous antiserum. Regarding collecting dates of antiserum, indirect ELISA revealed that antiserum of the first collecting date (after 2 days of the last injection) was the best comparing with those of second and the third dates (7 and 14 days respectively) .Titre of the obtained antiserum was 1:10.24X104 as determined by indirect ELISA. Indirect ELISA revealed the efficiency of this antiserum for comparing among the different bacterial isolates.

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