Identification of some faba bean (Vicia faba L.) varieties using electrophoresis protein and isozymes [electronic resource].

Includes reference.

The objectives of this study were to identify ten Faba bean (Vicia fuba L.) varieties using electrophoresis protein and some isozymes. The results of electrophoresis protein revealed that total number of bands ranged from 8 in variety G.643 to 12 in varieties G.461 and Sa2. Three common bands were found in all varieties, which have molecular weight of 84.742, 51.620 and 38.554 kda. Some varieties showed specific bands which could be used to distinguish between others. Each of Sa.1, M.1, Nu.1, G.843 and G.717 varieties had ouly unique band, with molecular weight of 223.041, 192.156, 141.056, 90.412 and 10.421kda, respectively. Band for MW of 30.746 kda is found in in the tested varieties except G.643, G.843 and Sa 1, which could be considered as a negative unique marker. Bands of three isozymes systems (peroxidase Prx, glutamate oxaloacetate transaminase GOT and esterase Est.) were determined for identification of faba bean varieties based on polyacrylamide gel electrophoresis. Using the number of bands and its Rfvalues can be used to identify these varieties. The number of GOT bands indicated that the varieties G.717 and Nu.l contained ouly four bands .To distinguish between these varieties upon RF values of these bands which have 0.125, 0.281, 0.346, 0.408 and 0.118,0.232,0.285,0.355, respectively. Esterase bands of various varieties indicated that the G. 3 Im variety have seven bands ouly which led to define Ibisvariety from the others . Also, Nu.1 variety contained five bands. Peroxides bands of varieties demonstrate !bat G.717 variety contained 5 bands which characterize it from the others. While, the other varieties containedfrom 2 to 3 bands which were differ in Rf values and' can distinguish between them depending on the difference of their Rf values.

Summary in Arabic.

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