Effect of BA, NAA and gamma irradiation on the production of three cultivars of carnation (Dianthus caryophyllus L.) through tissue culture [electronic resource].

Includes references.

This investigation was carried out on three cultivars of carnation plant (Medea, Candela and Picaro), cultured on solid MS medium supplemented with 0.5, 1.0, 2.0 or 3.0 mg/l of BA in combination with NAA at the rate of 0.0 0.25, 0.50 and l.0 mg/l. .After two months the optimal concentration of BA was chosen and explants were subcultured on shooting medium (MS) enriched with BA at (2.0 mg) and were exposed to gamma rays at 10, 20, 30 and 40 Gy. The results indicated that maximum number of shoots of CVS. Medea and Candela were produced on MS medium enriched with 2.0 and 3.0 mg/l of BA without NAA, whereas the maximum number of shoots of cv. Picaro was obtained on medium enriched with 2.0 BA alone followed by 2.0 BA + 0.25 mg/l NAA. The cultivar Medea formed significantly longer shoots compared with the other two CVS. The growing of the explants from Medea and Picaro cultivars on MS medium containing 2.0 mg/l BA + 0.25 NAA gave the longest shoots. Callus formation of the Medea and Picaro was the best on MS medium supplemented with 0.5 mg/l BA +1.0 mg/l NAA, however the addition of BA at 3.0 mg/l showed no callus formation. Callus formation of the Candela was greatly increased with the application of NAA at lmg/l +0.25 mg BA II. The highest survival percentage (78.68%) was recorded with the cultivar Medea. The addition of BA at 0.5, l.0 or 3.0 mg/l +0.5 NAA or BA at 2.0 mg/l+ 0.5 NAA to MS medium gave a significant increasing of the survival percentage and medium containing NAA alone reduced it to the minimum values. Exposing the explants of the three carnation cvs. to gamma irradiation at the different doses decreased the average shoot number. The low dose of gamma rays (10 Gy), significantly increased the average shoot length. The cultivar Picaro, showed a significant increase in the shoot length with gamma irradiation at 30 Gy. Also, there was a significant difference in the formation of roots for all the carnation cultivars. Most of the irradiation treatments without or with NAA decreased the number and the length of roots.

Summary in Arabic.

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